2FA8

Crystal Structure of the Putative Selenoprotein W-related family Protein from Agrobacterium tumefaciens

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97925, 0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	53.078, 69.306, 95.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.420 - 1.900
R-factor	0.2007
Rwork	0.198
R-free	0.24808
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.371
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.420	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.085	0.496
Number of reflections	29741
<I/σ(I)>	8.7	1.9
Completeness [%]	97.9	84.3
Redundancy	6.6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.2M potassium chloride, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.2M potassium chloride, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.2M potassium chloride, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K