2F9Z

Complex between the chemotaxis deamidase CheD and the chemotaxis phosphatase CheC from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.005
Spacegroup name	P 32
Unit cell lengths	66.080, 66.080, 161.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.211
Rwork	0.211
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xkr
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	32861
Completeness [%]	93.4	89.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30% PEG 4000, 0.1M Tris, 0.25M Lithium Sulfate, 0.1M Cesium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K