2F9W
Structure of the type III CoaA from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.667, 90.667, 179.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.880 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f9t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59606 | |
| <I/σ(I)> | 30.5 | 4.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.7 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | water, Tirs-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
