2F9K
Crystal structure of human FPPS in complex with Zoledronate and Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.270, 111.270, 69.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.760 - 2.060 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.25900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNX (2005) |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.079 | 0.382 |
| Number of reflections | 27286 | 2495 |
| <I/σ(I)> | 21.23 | 8.51 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 14.2 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 292 | 0.1M zinc acetate, 0.1M sodium acetate, 12% PEG 4000, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
