2F9H
The Crystal Structure of PTS System IIA Component from Enterococcus faecalis V583
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | SBC-3 |
Wavelength(s) | 0.9793, 0.9795 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 58.249, 58.249, 140.443 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.320 - 1.570 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.22900 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.320 | 1.630 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.054 | 0.332 |
Number of reflections | 38988 | |
<I/σ(I)> | 17.3 | 4.5 |
Completeness [%] | 98.7 | 88.4 |
Redundancy | 12.7 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M Sodium acetate pH 4.6, 4% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M Sodium acetate pH 4.6, 4% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M Sodium acetate pH 4.6, 4% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |