2F96
2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.879, 76.636, 61.666 |
| Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.090 |
| R-factor | 0.16013 |
| Rwork | 0.158 |
| R-free | 0.20448 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.345 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.125 | 0.441 |
| Number of reflections | 27229 | |
| <I/σ(I)> | 17.93 | 2.567 |
| Completeness [%] | 99.5 | 96.5 |
| Redundancy | 4.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | PEG 3350, 25% W/V, MG CHLORIDE 0.2 M BIS-TRIS 0.3M NDSB 256, , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
