2F8M

Ribose 5-phosphate isomerase from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.03492
Spacegroup name	P 21 21 2
Unit cell lengths	93.689, 136.230, 45.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.087
Rwork	0.208
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uj5
RMSD bond length	0.017
RMSD bond angle	1.625
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR (2.5)
Refinement software	REFMAC (refmac_5.2.0005 24/04/2001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.080	2.080
Rmerge	0.073	0.368
Number of reflections	32530
<I/σ(I)>	25.818	3.916
Completeness [%]	92.7	96.6
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.4 ul protein 8 mg/ml, 0.4 ul crystallization buffer 200mM NaCl, 0.6 mM KAu(CN)2, 20% PEG 3000,100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K