2F8L
Crystal structure of a putative class i s-adenosylmethionine-dependent methyltransferase (lmo1582) from listeria monocytogenes at 2.20 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9797, 0.9796, 1.0000 |
Spacegroup name | I 41 |
Unit cell lengths | 99.595, 99.595, 117.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.660 - 2.200 |
R-factor | 0.16769 |
Rwork | 0.165 |
R-free | 0.21100 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.585 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 23.650 | 23.650 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.072 | 0.041 | 0.582 |
Number of reflections | 28765 | ||
<I/σ(I)> | 7.3 | 14.5 | 1.2 |
Completeness [%] | 99.4 | 99.5 | |
Redundancy | 3.7 | 3.2 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 8 | 277 | 2.4M (NH4)2SO4, 0.1M TRIS, pH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |