2F8E
Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with uridylylated VPg protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.381, 94.381, 99.726 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.2369 |
| Rwork | 0.234 |
| R-free | 0.28426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Foot and Mouth disease Virus |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.095 | 0.455 |
| Number of reflections | 11795 | |
| <I/σ(I)> | 7.5 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 33% PEG4K, 0.2M ammonium acetate, 0.1M sodium citrate pH 5.6, 4% butyrolactone, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
