2F7V
Structure of acetylcitrulline deacetylase complexed with one Co
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-03-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9780,0.9761,0.9550 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.068, 95.429, 43.670 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 37.330 - 1.750 |
| R-factor | 0.1971 |
| Rwork | 0.197 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.330 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.052 | 0.355 |
| Number of reflections | 38233 | |
| <I/σ(I)> | 18.4 | 2.8 |
| Completeness [%] | 98.7 | 97 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 100 mM sodium acetate, 200 mM sodium formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
