2F7S
The crystal structure of human Rab27b bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.469, 64.023, 153.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.24765 |
| Rwork | 0.245 |
| R-free | 0.29248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oiv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 15658 | |
| <I/σ(I)> | 37.6 | 5.69 |
| Completeness [%] | 99.3 | 96.1 |
| Redundancy | 6.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 20%(v/v) isopropanol, 25%(v/v)PEG 4K, 0.1M sodium citrate, 0.1 M bistris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
