2F7P
Golgi alpha-mannosidase II complex with benzyl-mannostatin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.954, 109.874, 139.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.280 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.100 | 0.575 |
| Number of reflections | 266693 | |
| <I/σ(I)> | 16.6 | 2.7 |
| Completeness [%] | 97.7 | 79.8 |
| Redundancy | 6.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Tris, PEG 6000, MPD, NaCl, pH 7, vapor diffusion, hanging drop, temperature 298K |
