2F7P
Golgi alpha-mannosidase II complex with benzyl-mannostatin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.91860 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.954, 109.874, 139.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.330 - 1.280 |
R-factor | 0.166 |
Rwork | 0.166 |
R-free | 0.18000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hty |
RMSD bond length | 0.017 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.330 | 1.310 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.100 | 0.575 |
Number of reflections | 266693 | |
<I/σ(I)> | 16.6 | 2.7 |
Completeness [%] | 97.7 | 79.8 |
Redundancy | 6.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Tris, PEG 6000, MPD, NaCl, pH 7, vapor diffusion, hanging drop, temperature 298K |