2F7I
Human transthyretin (TTR) complexed with diflunisal analogues- TTR. 2',6'-Difluorobiphenyl-4-carboxylic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-01-05 |
| Detector | CCD-PXL-L600 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.236, 85.139, 64.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.500 - 1.600 |
| R-factor | 0.23415 |
| Rwork | 0.233 |
| R-free | 0.25191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.241 |
| Data reduction software | LSCALE |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.900 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 30717 | |
| Completeness [%] | 99.7 | 99.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | AMMONIUM SULFATE, 100 MM KCL, 1 MM EDTA, 10 MM SODIUM PHOSPHATE, SOAKED WITH THE INHIBITOR, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
