2F6K

Crystal Structure of Amidohydrorolase II; Northeast Structural Genomics Target LpR24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	105
Detector technology	CCD
Collection date	2005-07-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 43 2 2
Unit cell lengths	82.620, 82.620, 240.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.900 - 2.500
R-factor	0.192
Rwork	0.192
R-free	0.22500
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (V. 1.1A)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.118
Number of reflections	27059
<I/σ(I)>	10.6	3.7
Completeness [%]	90.5	88.8
Redundancy	6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2M Ammonium Sulfate, 0.01M Magnesium Acetate, 0.05M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K