2F6D
Structure of the complex of a glucoamylase from Saccharomycopsis fibuligera with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2002-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9096 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.580, 85.350, 97.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.11975 |
| Rwork | 0.118 |
| R-free | 0.15958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.609 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 | 0.144 |
| Number of reflections | 59266 | |
| <I/σ(I)> | 17.5 | 4.2 |
| Completeness [%] | 94.5 | |
| Redundancy | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 50 mM acetate buffer, 15% PEG 8K, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
