2F61

Crystal structure of partially deglycosylated acid beta-glucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-06-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	108.640, 284.430, 91.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.980 - 2.500
R-factor	0.202
Rwork	0.196
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1OGS
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.980	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.092	0.390
Number of reflections	41998
<I/σ(I)>	15.2	4.5
Completeness [%]	89.0	93
Redundancy	4.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	1.2-1.4 M Ammonium Sulphate, 96 mM Na-citrate, 10 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K