2F5W
Cross-linked barnase soaked in 3 M thiourea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 32 |
| Unit cell lengths | 59.500, 59.500, 81.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.001 |
| Rwork | 0.186 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f4y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.100 |
| High resolution limit [Å] | 2.001 | 2.001 |
| Number of reflections | 17459 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.2 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | PROTEIN 20 MG/ML, AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
