2F5U
Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2004-06-24 |
| Detector | SBC-2 |
| Wavelength(s) | .97948, .97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.600, 67.600, 119.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.100 - 2.100 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 191657 |
| Completeness [%] | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15-25% PEG MME 2000, 200mM Triethylamine N-oxide hydrate, 100mM Tris pH 8.0 to 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
