2F5O
MutM crosslinked to undamaged DNA sampling G:C base pair IC3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2003-09-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.543, 93.253, 105.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.630 - 2.050 |
Rwork | 0.203 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r2y |
RMSD bond length | 0.017 |
RMSD bond angle | 1.800 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.125 | 0.514 |
Number of reflections | 26907 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 92.9 | 89.4 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 77K, temperature 277K |