2F5N
MutM crosslinked to undamaged DNA sampling A:T base pair IC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2003-07-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.379, 92.947, 105.195 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.810 - 2.000 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r2y |
RMSD bond length | 0.025 |
RMSD bond angle | 2.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.094 | 0.484 |
Number of reflections | 29774 | |
<I/σ(I)> | 2.78 | |
Completeness [%] | 98.2 | 90.2 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |