2F5I
X-ray structure of spermidine/spermine N1-acetyltransferase (SAT) from Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 46.336, 46.336, 180.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.200 - 2.300 |
| R-factor | 0.2013 |
| Rwork | 0.201 |
| R-free | 0.26900 |
| Structure solution method | SIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.216 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.115 |
| Number of reflections | 15954 | |
| <I/σ(I)> | 18.9 | 6.7 |
| Completeness [%] | 95.2 | 72.8 |
| Redundancy | 3.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 300 | 3M NaCl , 0.1M TrisHCl, pH 8.0, VAPOR DIFFUSION, temperature 300K |
