2F5G
Crystal structure of IS200 transposase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.80000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.543, 68.149, 65.490 |
| Unit cell angles | 90.00, 129.24, 90.00 |
Refinement procedure
| Resolution | 26.550 - 1.700 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 34831 | |
| Completeness [%] | 97.5 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 297 | 1.4M tri-sodium citrate, 100mM sodium HEPES (pH 7.5), EVAPORATION, temperature 297K |
| 1 | EVAPORATION | 7.5 | 297 | 1.4M tri-sodium citrate, 100mM sodium HEPES (pH 7.5), EVAPORATION, temperature 297K |
