2F5E
Bacillus subtilis manganese transport regulator (MNTR) bound to manganese, AB conformation, pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.400, 46.010, 74.360 |
| Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.200 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.325 |
| Number of reflections | 16728 | |
| <I/σ(I)> | 7.7 | 2.2 |
| Completeness [%] | 97.3 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 15% PEG 4000, 0.1 M MAGNESIUM CHLORIDE, 0.1 M CACODYLATE, PH 6.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K |
