2F56
Barnase cross-linked with glutaraldehyde soaked in 6M urea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 32 |
| Unit cell lengths | 59.280, 59.280, 81.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.955 |
| Rwork | 0.162 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f4y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.100 |
| High resolution limit [Å] | 1.955 | 1.955 |
| Number of reflections | 21767 | |
| <I/σ(I)> | 22 | 10.7 |
| Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | AMMONIUM SULFATE, HEPES BUFFER. PROTEIN 20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
