2F4V

30S ribosome + designer antibiotic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Collection date	2002-06-01
Wavelength(s)	0.991
Spacegroup name	P 41 21 2
Unit cell lengths	403.323, 403.323, 176.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.800
R-factor	0.26179
Rwork	0.259
R-free	0.31456
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.635
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.930
High resolution limit [Å]	3.800	3.800
Rmerge	0.161	0.680
Number of reflections	138452
Completeness [%]	97.4	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1