2F4S

A-site RNA in complex with neamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Collection date	2002-06-01
Wavelength(s)	0.991
Spacegroup name	P 21 21 21
Unit cell lengths	33.055, 47.310, 85.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.249
Rwork	0.225
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.079	0.057	0.327
Number of reflections	3231
<I/σ(I)>	8.1		2.3
Completeness [%]	89.7	74.6	85.8
Redundancy	4.7	4.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1