2F4P
Crystal structure of a cupin-like protein (tm1010) from thermotoga maritima msb8 at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000, 0.9797 |
| Spacegroup name | P 65 |
| Unit cell lengths | 71.746, 71.746, 188.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.540 - 1.900 |
| R-factor | 0.164 |
| Rwork | 0.160 |
| R-free | 0.21600 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 21.540 | 21.540 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.083 | 0.058 | 0.549 |
| Number of reflections | 43148 | ||
| <I/σ(I)> | 6.1 | 9 | 1.4 |
| Completeness [%] | 99.9 | 99.9 | |
| Redundancy | 6.6 | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 4 | 277 | 1.0M LiCl, 20.0% PEG-6000, 0.1M Citrate, pH 4.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
