2F4G
Triclinic cross-linked lysozyme soaked in bromoethanol 1M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.130, 31.750, 34.190 |
| Unit cell angles | 88.96, 108.42, 111.47 |
Refinement procedure
| Resolution | 10.000 - 1.654 |
| Rwork | 0.178 |
| R-free | 0.18000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f2n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.800 |
| High resolution limit [Å] | 1.654 | 1.654 |
| Number of reflections | 10770 | |
| Completeness [%] | 90.1 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | SODIUM NITRATE AS CRYSTALLIZING AGENT CROSS-LINKED BY VAPOR DIFFUSION WITH GLUTARALDEHYDE, PROTEIN 20 MG/ML, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
