2F44
Crystal Structure of the Zinc-bound Shank SAM domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-03-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.28250 |
Spacegroup name | P 65 |
Unit cell lengths | 104.820, 104.820, 40.192 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f3n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 9135 | |
<I/σ(I)> | 14.5 | 2.2 |
Completeness [%] | 89.4 | 67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | Well Solution: 0.1 M HEPES pH 7.2, 0.4 M Ammonium Formate, 6.5 mM N-nonyl BD glucoside. Protein Buffer: 5 mM Tris pH 8.0, 50 mM NaCl, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |