2F42

dimerization and U-box domains of Zebrafish C-terminal of HSP70 interacting protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-05
Detector	NOIR-1
Wavelength(s)	1.2399, 0.9786
Spacegroup name	P 65 2 2
Unit cell lengths	100.499, 100.499, 74.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.250 - 2.500
R-factor	0.266
Rwork	0.266
R-free	0.28500
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	d*TREK
Data scaling software	d*TREK (9.4LDz)
Phasing software	SOLVE (2.06)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.250	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.052	0.480
Number of reflections	8099
<I/σ(I)>	24.5	5.7
Completeness [%]	100.0	100
Redundancy	19.12	18.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	9% PEG3350, 100mM Bis-Tris, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K