2F41
Crystal structure of FapR- a global regulator of fatty acid biosynthesis in B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.07175 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.239, 84.320, 155.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.22162 |
| Rwork | 0.219 |
| R-free | 0.27377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.826 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.170 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.083 | 0.287 |
| Number of reflections | 14880 | |
| <I/σ(I)> | 13.7 | 4.8 |
| Completeness [%] | 80.5 | 50 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.2M ammonium sulfate, 2% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
