2F30
Triclinic cross-linked Lysozyme soaked with 4.5M urea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.110, 31.940, 34.170 |
| Unit cell angles | 88.15, 108.62, 111.73 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| R-factor | 0.1564 |
| Rwork | 0.150 |
| R-free | 0.19100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f2n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SFALL |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.300 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.039 | 0.195 |
| Number of reflections | 11365 | |
| <I/σ(I)> | 28.3 | 18 |
| Completeness [%] | 98.4 | 85.6 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | sodium nitrate as crystallizing agent cross-linked by vapor diffusion with glutaraldehyde, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
