Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.700, 62.700, 287.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.810 - 2.100 |
| R-factor | 0.17306 |
| Rwork | 0.170 |
| R-free | 0.23355 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.476 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.02) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.810 | 50.000 | 2.150 |
| High resolution limit [Å] | 2.080 | 4.480 | 2.080 |
| Rmerge | 0.065 | 0.058 | 0.260 |
| Number of reflections | 30515 | ||
| <I/σ(I)> | 22.303 | 6.376 | |
| Completeness [%] | 84.9 | 92 | 44.8 |
| Redundancy | 9.5 | 9.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Protein solution (15 mg/ml protein, 0.025 M sodium chloride, 0.10 M Tris pH 7.5) mixed in a 1:1 ratio with the well solution (0.055 M sodium acetate pH 4.5, 1.05 M ammonium sulfate), temperature 277 K, VAPOR DIFFUSION, HANGING DROP |
