2F2B
Crystal structure of integral membrane protein Aquaporin AqpM at 1.68A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2005-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 88.513, 88.513, 79.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.680 |
| R-factor | 0.193 |
| Rwork | 0.184 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2evu |
| RMSD bond angle | 0.008 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.078 | 0.590 |
| Number of reflections | 34031 | |
| <I/σ(I)> | 18.8 | 3.3 |
| Completeness [%] | 96.8 | 83.7 |
| Redundancy | 9.1 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG4000 23%, 200 mM MgCl2, 10% glycerol, TRIS pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
