2F26
Crystal Structure of the Human Sialidase Neu2 E111Q-Q112E Double Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-05 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 0.9780 |
| Spacegroup name | H 3 |
| Unit cell lengths | 145.650, 145.650, 64.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.550 - 1.580 |
| Rwork | 0.209 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1snt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.550 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.053 | 0.351 |
| Number of reflections | 69226 | |
| <I/σ(I)> | 18.9 | 6.6 |
| Completeness [%] | 99.5 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | sodium potassium phosphate, sodium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
