2F1K

Crystal structure of Synechocystis arogenate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-12
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	66.150, 70.850, 104.490
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	20.000 - 1.550
R-factor	0.1903
Rwork	0.189
R-free	0.22191
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.665
Data scaling software	XDS
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	135727
<I/σ(I)>	17.06	3.01
Completeness [%]	97.0	85.5
Redundancy	4.09	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	294	1 uL prot + 1 uL reservoir (PEG 6000 30%, 0.1 M tris-HCl) + 2uL NADP 2 mM, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 8.00
1		8	294	1 uL prot + 1 uL reservoir (PEG 6000 30%, 0.1 M tris-HCl) + 2uL NADP 2 mM, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 8.00
1		8	294	1 uL prot + 1 uL reservoir (PEG 6000 30%, 0.1 M tris-HCl) + 2uL NADP 2 mM, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 8.00