2F1J
Recombinase in Complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-16 |
| Detector | ENRAF-NONIUS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 82.974, 82.974, 105.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.24400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 |
| Data reduction software | LSCALE |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 18093 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M MAGNESIUM CHLORIDE, 0.5 M K Cl, 0.05 M TRIS, 6% PEG 3350 , pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
