2F1C
Crystal structure of the monomeric porin OmpG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.640, 70.142, 59.501 |
| Unit cell angles | 90.00, 104.06, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.237 |
| Rwork | 0.233 |
| R-free | 0.26000 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.121 | 0.475 |
| Number of reflections | 19961 | |
| <I/σ(I)> | 25.7 | 2.8 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 6.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 295 | 28-32% PEG400, 1-1.5 M sodium formate, 50 mM cacodylate pH 5.5, VAPOR DIFFUSION, temperature 295K |
