2F15
Glycogen-Binding Domain Of The Amp-Activated Protein Kinase beta2 Subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 69.723, 69.723, 118.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.750 - 2.000 |
| R-factor | 0.23923 |
| Rwork | 0.237 |
| R-free | 0.29608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z0m |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.750 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.547 |
| Number of reflections | 7696 | |
| <I/σ(I)> | 59.8 | 2.8 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 9.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 27% MPD, 0.1 M Hepes, pH 7.5, 0.2 M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
