2F0V

Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	48.139, 48.139, 63.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.1895
Rwork	0.189
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.021
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.000
Number of reflections	9249

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K