2F0P

Crystal structure of Staphylococcal nuclease mutant V66I/V99I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	48.062, 48.062, 63.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.050
R-factor	0.19091
Rwork	0.191
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.022
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.050
Number of reflections	8549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	50mM phosphate buffer, 45% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K