2F01

Epi-biotin complex with core streptavidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.54178
Spacegroup name	I 2 2 2
Unit cell lengths	46.390, 93.811, 104.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 0.850
R-factor	0.1562
Rwork	0.155
R-free	0.17360
Structure solution method	isomorphous with known structure
Starting model (for MR)	1mk5
RMSD bond length	0.018
RMSD bond angle	0.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	0.860
High resolution limit [Å]	0.850	0.850
Number of reflections	189859
Completeness [%]	95.0	83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	277	38% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.5, 0.2 M sodium chloride, VAPOR DIFFUSION, temperature 277K
1	VAPOR DIFFUSION	4.5	277	38% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.5, 0.2 M sodium chloride, VAPOR DIFFUSION, temperature 277K