2F00
Escherichia coli MurC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790, 0.9792, 0.8377 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.926, 93.134, 176.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.000 - 2.500 |
| Rwork | 0.217 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.139 | |
| Number of reflections | 42732 | |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 12-16% mPEG5000, 0.2 M Bis-Tris Propane pH 8.3, 10 mM MgCl2, 50 mM beta-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
