2EYU
The Crystal Structure of the C-terminal Domain of Aquifex aeolicus PilT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 41 |
| Unit cell lengths | 89.150, 89.150, 70.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.870 |
| Rwork | 0.170 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.940 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.080 | 0.197 |
| Number of reflections | 44351 | |
| <I/σ(I)> | 49.9 | 7.8 |
| Completeness [%] | 96.8 | 83.9 |
| Redundancy | 12 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mg/ml protein 0.1 M Mes 10% Dioxane 1.6 M ammonium sulfate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
