2EYN

Crystal structure of the actin-binding domain of human alpha-actinin 1 at 1.8 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-29
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	53.007, 111.066, 31.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
Rwork	0.184
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human alpha-actinin 1 ABD PDB 2EYI
RMSD bond length	0.007
RMSD bond angle	1.280
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.780	1.780
Rmerge	0.067	0.283
Number of reflections	17130
<I/σ(I)>	32.7	3.5
Completeness [%]	92.4	61.6
Redundancy	5.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	277.5	50mM NaCl, 1mM EDTA, 100mM imidazol, 24% PEG 5000 MME, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277.5K