2EYI

Crystal structure of the actin-binding domain of human alpha-actinin 1 at 1.7 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-29
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	56.763, 110.899, 34.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.700
Rwork	0.183
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CH1 of plectin ABD (pdb entry 1MB8) CH2 of spectrin ABD (pdb entry 1AA2)
RMSD bond length	0.009
RMSD bond angle	1.190
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.077	0.292
Number of reflections	23591
<I/σ(I)>	53.5	6.6
Completeness [%]	94.1	69
Redundancy	10.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	279.5	50mM NaCl, 1mM EDTA, 100mM imidazol, 24% polyethylene glycol 5000 monomethyl ester (PEG 5000 MME) , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 279.5K