2EXW
Crystal structure of a EcClC-Fab complex in the absence of bound ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.919464 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 219.834, 122.436, 151.344 |
Unit cell angles | 90.00, 128.16, 90.00 |
Refinement procedure
Resolution | 19.980 - 3.200 |
R-factor | 0.268 |
Rwork | 0.268 |
R-free | 0.31400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ots |
RMSD bond length | 0.010 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.100 | |
Number of reflections | 56184 | |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.0 | 88.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | peg 200/300 1:2, 150mM NaKTart, 50mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |