2EX5
Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.234, 69.176, 169.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | molecular replacement was performed with a starting model generated from the lower resolution MAD phases. |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.324 |
| Number of reflections | 29588 | |
| <I/σ(I)> | 25.2 | 3.2 |
| Completeness [%] | 96.0 | 100 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 26-36% (v:v) PEG 400, 100 mM TrisHCl, 100 mM KCl, 10 mM CaCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 26-36% (v:v) PEG 400 | ||
| 2 | 1 | 1 | 100 mM TrisHCl | ||
| 3 | 1 | 1 | 100 mM KCl | ||
| 4 | 1 | 1 | 10 mM CaCl2 | ||
| 5 | 1 | 2 | 26-36% (v:v) PEG 400 | ||
| 6 | 1 | 2 | 100 mM KCl | ||
| 7 | 1 | 2 | 10 mM CaCl2 |
