2EX3
Bacteriophage phi29 DNA polymerase bound to terminal protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 304.933, 220.281, 217.165 |
Unit cell angles | 90.00, 45.40, 90.00 |
Refinement procedure
Resolution | 48.850 - 3.000 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A lower resolution partially refined complex determined by heavy atom phasing |
RMSD bond length | 0.007 |
RMSD bond angle | 1.002 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.085 | 0.000 |
Number of reflections | 202295 | |
<I/σ(I)> | 1.25 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Polymerase (6.7 mg/ml), terminal protein (3.3 mg/ml) in 50 mM NaCl, 50 mM Tris-HCl pH 7.5, 20 mM Ammonium sulfate, 1 mM dithiothreitol, mixed with an equal volume of well solution containing: 2.0 M sodium/potassium phosphate, pH 7, 100 mM sodium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 277K |