2EWT
Crystal structure of the DNA-binding domain of BldD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-05 |
| Detector | MAC Science DIP-2030B |
| Wavelength(s) | 0.97932, 0.97942, 0.98722 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.199, 31.774, 33.644 |
| Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
| Resolution | 19.570 - 1.810 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 7031 | |
| Completeness [%] | 94.5 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 25% PEG 4000, 0.1M sodium acetate (pH 4.6), 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
